The Influence of Extracts from Common Houseleek (Sempervivum tectorum) on the Metabolic Activity of Human Melanoma Cells WM-266-4

Human melanoma cells are known as one of the most aggressive cancer cells, and consequently, is incurable diseases. There intense activity in research development potential medicines for malignant diseases, including alternative forms remedies. Therefore, purpose our work was testing extracts from common houseleek (Sempervivum tectorum) grown Slovenia to establish its impact on human cells. Namely, we wanted verify if inhibit growth their metabolic activity. Soxhlet, cold solvent, ultrasound, supercritical extraction methods were applied obtain S. tectorum extracts. Polyphenols proanthocyanins content acquired determined well antioxidative potential. For a relevant comparison, Chinese (CHI) dried Slovenian (SLO) lyophilized used. Results showed that highest contents polyphenols yielded material, which also had The focus analysis possible inhibition effects since no past studies found regarding WM-266-4. We established 24-h incubation period, inhibited at concentrations 20, 10, 4, 2, 1, 0.2, 0.02 mg/mL. Extract obtained SLO (ultrasound with acetone solvent), promising results inhibitory effect mentioned further described local plant study. CHI extract (Soxhlet ehtanol:water mixture = 1:1 solvent) WM-266-4, although both suitable this specific cell line. Our suggest ability WM-266-4 metastatic line, suggesting use an anticancer agent.